Facility Overview: Cell Processing Center (CPC)

The facility is structured as a controlled environment specifically designed for the handling, cultivation, and analysis of cellular products. The layout follows a logical flow from personnel preparation to specialized laboratory zones.

1. Entry and Gown Zones (Preparation)

• Main Entrance & Anteroom: The initial point of entry where the transition from the external environment to the controlled zone begins.

• 1st & 2nd Dressing Rooms: A two-stage "gowning" process to ensure strict contamination control. Staff change into specialized cleanroom attire before proceeding through the Corridor to the sterile work areas.

2. Core Processing Zones

• Cultivation Room: The heart of the center, equipped with specialized stations for cell manipulation:

  • Cell Centrifuge: For isolating specific cell populations.

  • Microscopes (Scope): For real-time observation of cell health and morphology.

  • Incubators: For stable cell growth.

  • FACS (Cell Counter): Fluorescence-activated cell sorting for high-precision analysis and quantification.

• Cell Preservation Room: Dedicated space for the short-term and long-term storage of processed cells.

• Deep Refrigerator: High-performance cryogenic storage units to maintain cell viability at ultra-low temperatures.

3. Support and Quality Control

• Testing & Measuring Room: A large diagnostic area equipped with multiple computer workstations and analytical instruments to monitor experimental data and ensure quality assurance.

• Materials Room: A dedicated space for storing sterile consumables, reagents, and necessary laboratory supplies.

• Passbox: A specialized transfer hatch that allows materials to be moved between rooms without personnel movement, minimizing the risk of cross-contamination.

4. Environmental Control System (HVAC)

The facility features a sophisticated air management system to maintain cleanroom integrity:

• SA (Supply Air): Filtered air pumped into the rooms to maintain positive pressure.

• RA (Return Air) & EA (Exhaust Air): Systems that remove contaminated or used air.

• OA (Outside Air): Fresh air intake filtered through the primary mechanical system.

• Exhaust System: Specifically designed to vent air safely, ensuring a constant directional airflow that prevents airborne particles from entering sterile zones.

Key Feature: The Watch Window allows for external observation of the Cultivation Room, enabling supervision or tours without compromising the sterile environment.